IgG4 anti-neurofascin155 antibodies in chronic inflammatory demyelinating polyradiculoneuropathy: Clinical significance and diagnostic utility of a conventional assay

Masato Kadoya; Kenichi Kaida; Haruki Koike; Hiroshi Takazaki; Hidenori Ogata; Kota Moriguchi; Jun Shimizu; Eiichiro Nagata; Shunya Takizawa; Atsuro Chiba; Ryo Yamasaki; Jun-Ichi Kira; Gen Sobue; Katsunori Ikewaki

doi:10.1016/j.jneuroim.2016.10.013

IgG4 anti-neurofascin155 antibodies in chronic inflammatory demyelinating polyradiculoneuropathy: Clinical significance and diagnostic utility of a conventional assay

J Neuroimmunol. 2016 Dec 15:301:16-22. doi: 10.1016/j.jneuroim.2016.10.013. Epub 2016 Nov 9.

Authors

Affiliations

¹ Department of Neurology, Anti-aging and Vascular Medicine, National Defense Medical College, Tokorozawa, Saitama 359-8513, Japan.
² Department of Neurology, Anti-aging and Vascular Medicine, National Defense Medical College, Tokorozawa, Saitama 359-8513, Japan. Electronic address: adiak901@ndmc.ac.jp.
³ Department of Neurology, Nagoya University Graduate School of Medicine, Nagoya, Aichi 466-8550, Japan.
⁴ Department of Neurology, Neurological Institute, Graduate School of Medical Sciences, Kyushu University, Higashi-Ku, Fukuoka 812-8582, Japan.
⁵ Department of Neurology, University of Tokyo, Bunkyo-Ku, Tokyo 113-8655, Japan.
⁶ Department of Neurology, Tokai University School of Medicine, Isehara, Kanagawa 259-1193, Japan.
⁷ Department of Neurology, Kyorin University, Mitaka, Tokyo 181-8611, Japan.

PMID: 27852440
DOI: 10.1016/j.jneuroim.2016.10.013

Abstract

We aimed to validate the diagnostic utility of enzyme-linked immunosorbent assay (ELISA) for the detection of anti-neurofascin (NF) 155 antibody in 191 patients with chronic inflammatory demyelinating polyneuropathy (CIDP). Human NF155-based ELISA clearly distinguished between anti-NF155 antibody-positive and -negative sera. Fifteen CIDP patients (8%) were IgG4 anti-human NF155 antibody-positive, which were confirmed by western blot, cell-based assay and immunohistochemical study. None of disease controls or healthy subjects had positive results. Clinical presentation of IgG4 anti-NF155 antibody-positive patients was consistent with those in previous reports. This ELISA combined with determination of the IgG4 subclass is useful in screening for anti-NF155 antibodies.

Keywords: Antibody; Chronic inflammatory demyelinating polyradiculoneuropathy; Enzyme-linked immunosorbent assay; IgG4; Neurofascin155.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adolescent
Adult
Aged
Animals
Cell Adhesion Molecules / genetics
Cell Adhesion Molecules / immunology*
Cell Adhesion Molecules, Neuronal / metabolism
Enzyme-Linked Immunosorbent Assay
Female
Flow Cytometry
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
HEK293 Cells
Humans
Immunoglobulin G / blood*
Male
Middle Aged
Nerve Growth Factors / genetics
Nerve Growth Factors / immunology*
Polyradiculoneuropathy, Chronic Inflammatory Demyelinating / blood*
Polyradiculoneuropathy, Chronic Inflammatory Demyelinating / immunology
Polyradiculoneuropathy, Chronic Inflammatory Demyelinating / pathology
Rats
Sciatic Nerve / metabolism
Statistics, Nonparametric
Transfection
Young Adult

Substances

CNTNAP1 protein, human
Cell Adhesion Molecules
Cell Adhesion Molecules, Neuronal
Immunoglobulin G
NFASC protein, human
Nerve Growth Factors
Green Fluorescent Proteins