Quantitation of Mouse Monoclonal Antibody and Human Anti-Mouse Antibody Response in Serum of Patients

Hybridoma. 1989 Apr;8(2):231-9. doi: 10.1089/hyb.1989.8.231.

Abstract

The assays required to study pharmacokinetics of monoclonal antibodies and human anti-mouse antibody response in man, utilizing enzyme linked immunosorbent assay, have been at best semi-quantitative. We have developed and well characterized a quantitative assay for measurement of murine monoclonal antibodies and human anti-mouse antibody in circulation of patients injected with murine monoclonal antibodies. The sensitivity of mouse IgG assay is approximately 1 ng/ml and cross reactivity of IgG's from other species were less than 0.01%. The pre-therapy samples from 30 patients treated with monoclonal antibody CO17-1A as well as serum from 54 individuals had no detectable human anti-mouse antibody indicating specificity. The sensitivity of this assay was approximately 10 mg/ml. The utility of these assays was demonstrated in a group of patients receiving 400 mg of monoclonal antibody CO17-1A. The half-life and the degree of immune response can be measured using these assays.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Anti-Idiotypic / biosynthesis*
  • Antibodies, Monoclonal / analysis*
  • Antibodies, Monoclonal / therapeutic use
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Immunoglobulin G / analysis
  • Kinetics
  • Mice

Substances

  • Antibodies, Anti-Idiotypic
  • Antibodies, Monoclonal
  • Immunoglobulin G