Zika Virus: Quantification, Propagation, Detection, and Storage

doi:10.1002/cpmc.19

. 2016 Nov 18:43:15D.4.1-15D.4.16.

doi: 10.1002/cpmc.19.

Zika Virus: Quantification, Propagation, Detection, and Storage

Darrell S Agbulos¹, Larissa Barelli^{1

2}, Bryan V Giordano¹, Fiona F Hunter^{1

2

3}

Affiliations

¹ Centre for Biotechnology, Brock University, St. Catharines, Ontario, Canada.
² Entomogen Incorporated, St. Catharines, Ontario, Canada.
³ Department of Biological Sciences, Brock University, St. Catharines, Ontario, Canada.

PMID: 27858969
DOI: 10.1002/cpmc.19

Zika Virus: Quantification, Propagation, Detection, and Storage

Darrell S Agbulos et al. Curr Protoc Microbiol. 2016.

. 2016 Nov 18:43:15D.4.1-15D.4.16.

doi: 10.1002/cpmc.19.

Authors

Darrell S Agbulos¹, Larissa Barelli^{1

2}, Bryan V Giordano¹, Fiona F Hunter^{1

2

3}

Affiliations

¹ Centre for Biotechnology, Brock University, St. Catharines, Ontario, Canada.
² Entomogen Incorporated, St. Catharines, Ontario, Canada.
³ Department of Biological Sciences, Brock University, St. Catharines, Ontario, Canada.

PMID: 27858969
DOI: 10.1002/cpmc.19

Abstract

Zika virus (ZIKV), belonging to the family Flaviviridae, genus Flavivirus, is an arthropod-borne virus that was first discovered from the Zika forest in Uganda in 1947. Recent outbreaks in South America have linked ZIKV to cases of microcephaly and Guillain-Barré syndrome in humans. With the increased interest in ZIKV, protocols must be established to facilitate proper research. Here we describe the laboratory techniques required to quantify, propagate, and store ZIVK. We also review the proper safety protocol for the handling of ZIKV, which is classified as a Biosafety Level 2 pathogen by the United States Centers for Disease Control and Prevention. © 2016 by John Wiley & Sons, Inc.

Keywords: Vero cells; Zika virus; detection; infection; plaque assay.

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References

Literature Cited

1. Akoua-Koffi, C., Diarrassouba, S., Bénié, V.B., Ngbichi, J.M., Bozoua, T., and Bosson, A. 2001. Investigation surrounding a fatal case of yellow fever in Côte d'Ivoire in 1999. Bull. Soc. Pathol. Exot. 94:227-230.
1. Ammerman, N.C., Beier-Sexton, M., and Azad, A.F. 2008. Growth and maintenance of vero cell lines. Curr. Protoc. Micro. 11:A.4E.1-A.4E.7. doi: 10.1002/9780471729259.mca04es11.
1. Atkinson, B., Hearn, P., Afrough, B., Lumley, S., Carter, D., Aarons, E.J., Simpson, A.J., Brooks, T.J., and Hewson, R. 2016. Detection of Zika virus in semen. Emerg. Infect. Dis. 22:940. doi: 10.3201/eid2205.160107.
1. Brien, J.D., Lazear, H.M., and Diamond, M.S. 2013. Propagation, quantification, detection, and storage of West Nile virus. Curr. Protoc. Microbiol. 31:15D.3.1-15D.3.18. doi: 10.1002/9780471729259.mc15d03s31.
1. Caraguel, C.G.B, Stryhn, H., Gagné, N., Dohoo, I.R., and Hammell, L. 2011. Selection of a cutoff value for real-time polymerase chain reaction results to fit a diagnostic purpose: Analytical and epidemiologic approaches. J. Vet. Diagn. Invest. 23:2-15. doi: 10.1177/104063871102300102.

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[1] Akoua-Koffi, C., Diarrassouba, S., Bénié, V.B., Ngbichi, J.M., Bozoua, T., and Bosson, A. 2001. Investigation surrounding a fatal case of yellow fever in Côte d'Ivoire in 1999. Bull. Soc. Pathol. Exot. 94:227-230.

[2] Akoua-Koffi, C., Diarrassouba, S., Bénié, V.B., Ngbichi, J.M., Bozoua, T., and Bosson, A. 2001. Investigation surrounding a fatal case of yellow fever in Côte d'Ivoire in 1999. Bull. Soc. Pathol. Exot. 94:227-230.

[3] Ammerman, N.C., Beier-Sexton, M., and Azad, A.F. 2008. Growth and maintenance of vero cell lines. Curr. Protoc. Micro. 11:A.4E.1-A.4E.7. doi: 10.1002/9780471729259.mca04es11.

[4] Ammerman, N.C., Beier-Sexton, M., and Azad, A.F. 2008. Growth and maintenance of vero cell lines. Curr. Protoc. Micro. 11:A.4E.1-A.4E.7. doi: 10.1002/9780471729259.mca04es11.

[5] Atkinson, B., Hearn, P., Afrough, B., Lumley, S., Carter, D., Aarons, E.J., Simpson, A.J., Brooks, T.J., and Hewson, R. 2016. Detection of Zika virus in semen. Emerg. Infect. Dis. 22:940. doi: 10.3201/eid2205.160107.

[6] Atkinson, B., Hearn, P., Afrough, B., Lumley, S., Carter, D., Aarons, E.J., Simpson, A.J., Brooks, T.J., and Hewson, R. 2016. Detection of Zika virus in semen. Emerg. Infect. Dis. 22:940. doi: 10.3201/eid2205.160107.

[7] Brien, J.D., Lazear, H.M., and Diamond, M.S. 2013. Propagation, quantification, detection, and storage of West Nile virus. Curr. Protoc. Microbiol. 31:15D.3.1-15D.3.18. doi: 10.1002/9780471729259.mc15d03s31.

[8] Brien, J.D., Lazear, H.M., and Diamond, M.S. 2013. Propagation, quantification, detection, and storage of West Nile virus. Curr. Protoc. Microbiol. 31:15D.3.1-15D.3.18. doi: 10.1002/9780471729259.mc15d03s31.

[9] Caraguel, C.G.B, Stryhn, H., Gagné, N., Dohoo, I.R., and Hammell, L. 2011. Selection of a cutoff value for real-time polymerase chain reaction results to fit a diagnostic purpose: Analytical and epidemiologic approaches. J. Vet. Diagn. Invest. 23:2-15. doi: 10.1177/104063871102300102.

[10] Caraguel, C.G.B, Stryhn, H., Gagné, N., Dohoo, I.R., and Hammell, L. 2011. Selection of a cutoff value for real-time polymerase chain reaction results to fit a diagnostic purpose: Analytical and epidemiologic approaches. J. Vet. Diagn. Invest. 23:2-15. doi: 10.1177/104063871102300102.

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Zika Virus: Quantification, Propagation, Detection, and Storage

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Zika Virus: Quantification, Propagation, Detection, and Storage

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