Inositol 1,3,4,5-tetrakisphosphate causes release of Ca2+ from permeabilized mouse lymphoma L1210 cells by its conversion into inositol 1,4,5-trisphosphate

P J Cullen; R F Irvine; B K Drøbak; A P Dawson

doi:10.1042/bj2590931

Inositol 1,3,4,5-tetrakisphosphate causes release of Ca2+ from permeabilized mouse lymphoma L1210 cells by its conversion into inositol 1,4,5-trisphosphate

Biochem J. 1989 May 1;259(3):931-3. doi: 10.1042/bj2590931.

Authors

P J Cullen¹, R F Irvine, B K Drøbak, A P Dawson

Affiliation

¹ School of Biological Sciences, University of East Anglia, Norwich, U.K.

Abstract

Addition of Ins(1,3,4,5)P4 at micromolar concentrations causes release of Ca2+ from electroporated L1210 cells, but not from digitonin-permeabilized cells. This was shown to be due to its conversion into Ins(1,4,5)P3, because only the electroporated cells convert Ins(1,3,4,5)P4 into Ins(1,4,5)P3. Thus electroporation appears to activate or expose an Ins(1,3,4,5)P4 3-phosphatase.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Calcium / metabolism*
Cell Membrane Permeability
Inositol 1,4,5-Trisphosphate
Inositol Phosphates / metabolism*
Inositol Phosphates / pharmacology*
Leukemia L1210 / metabolism*
Mice
Sugar Phosphates / metabolism*
Sugar Phosphates / pharmacology*
Tumor Cells, Cultured / drug effects

Substances

Inositol Phosphates
Sugar Phosphates
inositol-1,3,4,5-tetrakisphosphate
Inositol 1,4,5-Trisphosphate
Calcium