Randomized Prospective Double-Blind Studies to Evaluate the Cognitive Effects of Inositol-Stabilized Arginine Silicate in Healthy Physically Active Adults

Nutrients. 2016 Nov 18;8(11):736. doi: 10.3390/nu8110736.


Inositol-stabilized arginine silicate (ASI; Nitrosigine®) has been validated to increase levels of arginine, silicon and nitric oxide production. To evaluate potential enhancement of mental focus and clarity, ASI (1500 mg/day) was tested in two double-blind placebo-controlled crossover (DBPC-X) studies using the Trail Making Test (TMT, Parts A and B). In the two studies, healthy males took ASI for 14 and 3 days, respectively. In the first study, after 14 days of dosing, TMT B time decreased significantly from baseline (28% improvement, p = 0.045). In the second study evaluating shorter-term effects, TMT B time decreased significantly compared to placebo (33% improvement, p = 0.024) in a 10-min period. After 3 days of dosing, TMT B time significantly decreased from baseline scores (35% improvement, p < 0.001). These findings show that ASI significantly improved the ability to perform complex cognitive tests requiring mental flexibility, processing speed and executive functioning.

Keywords: ">l-arginine; cognitive function; mental flexibility; nitric oxide; silicate.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Adult
  • Arginine / administration & dosage*
  • Arginine / analogs & derivatives
  • Attention / drug effects
  • Cognition / drug effects*
  • Double-Blind Method
  • Drug Combinations
  • Executive Function / drug effects
  • Exercise*
  • Healthy Lifestyle*
  • Humans
  • Inositol / administration & dosage*
  • Male
  • Nootropic Agents / administration & dosage*
  • Prospective Studies
  • Silicates / administration & dosage*
  • Time Factors
  • Trail Making Test
  • Young Adult


  • Drug Combinations
  • Nootropic Agents
  • Silicates
  • nitrosigine
  • Inositol
  • Arginine