mScarlet: A Bright Monomeric Red Fluorescent Protein for Cellular Imaging

Nat Methods. 2017 Jan;14(1):53-56. doi: 10.1038/nmeth.4074. Epub 2016 Nov 21.

Abstract

We report the engineering of mScarlet, a truly monomeric red fluorescent protein with record brightness, quantum yield (70%) and fluorescence lifetime (3.9 ns). We developed mScarlet starting with a consensus synthetic template and using improved spectroscopic screening techniques; mScarlet's crystal structure reveals a planar and rigidified chromophore. mScarlet outperforms existing red fluorescent proteins as a fusion tag, and it is especially useful as a Förster resonance energy transfer (FRET) acceptor in ratiometric imaging.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteria / metabolism*
  • Bacterial Proteins / metabolism*
  • Bone Neoplasms / metabolism
  • Bone Neoplasms / pathology
  • Cell Survival
  • Fluorescence Resonance Energy Transfer / methods*
  • HeLa Cells
  • Humans
  • Luminescent Proteins / metabolism*
  • Molecular Imaging / methods*
  • Osteosarcoma / metabolism
  • Osteosarcoma / pathology
  • Protein Engineering / methods*
  • Tumor Cells, Cultured

Substances

  • Bacterial Proteins
  • Luminescent Proteins
  • red fluorescent protein