1-Oleoyl-2-acetylglycerol promotes immunoglobulin production independent of cell proliferation in human peripheral blood mononuclear cells

Biochem Biophys Res Commun. 1989 Jun 30;161(3):1319-26. doi: 10.1016/0006-291x(89)91387-9.

Abstract

1-Oleoyl-2-acetylglycerol (OAG) stimulated IgG and IgM production in a dose-dependent manner in human peripheral blood mononuclear cells (PBM) but not PBM proliferation. 12-O-Tetradecanoyl phorbol-13-acetate (TPA) did not stimulate Ig production. OAG did not stimulate an increase in IL-2 generation or IL-2 receptor expression. H-7, a protein kinase C blocker completely inhibited OAG-stimulated Ig production. The results suggest that OAG stimulation of Ig production is independent of cell proliferation; a generalized increase in T-cell activation does not appear to be necessary in the OAG stimulation of Ig production. Finally, PBMs respond differently to OAG and TPA although both are protein kinase C activators.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
  • Antibody Formation / drug effects
  • Cells, Cultured
  • DNA Replication / drug effects
  • Diglycerides / pharmacology*
  • Glycerides / pharmacology*
  • Humans
  • Immunoglobulin G / biosynthesis*
  • Immunoglobulin M / biosynthesis*
  • Interleukin-2 / biosynthesis
  • Isoquinolines / pharmacology
  • Lymphocytes / drug effects
  • Lymphocytes / immunology*
  • Piperazines / pharmacology
  • Protein Kinase Inhibitors
  • Receptors, Interleukin-2 / biosynthesis

Substances

  • Diglycerides
  • Glycerides
  • Immunoglobulin G
  • Immunoglobulin M
  • Interleukin-2
  • Isoquinolines
  • Piperazines
  • Protein Kinase Inhibitors
  • Receptors, Interleukin-2
  • 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
  • 1-oleoyl-2-acetylglycerol