Although molecular methods can now provide fast identification and the antibiotic susceptibility profile of infecting pathogens, these techniques are not affordable by a large majority of clinical laboratories in developing countries and can be considered excessive for simple, community-acquired infections. Most antibiotics are therefore prescribed empirically, which tends to avoid those drugs that face high resistance prevalence but that could still be used in a significant number of patients. This is a description of the basis for a fast, cheap method for assessing the presence of bacterial infection and its susceptibility to antibiotics in body fluids that are normally sterile, such as urine. The method is based on detecting a decreased concentration of glucose in liquid media as an indicator of bacterial growth, and the effect of antibiotics upon this change as an indicator of growth arrest by such antibiotics. The method can assess the presence of ca. 105CFU/mL in 4-6h as well as the presence of antibiotic resistance phenotypes of clinical relevance. The method was tested on 20 clinical urine samples, comparing its results with conventional culture and susceptibility assays; results of both the proposed method and the culture/antibiogram were in agreement in all cases. Materials needed for this method would cost no more than US$5 per assay, it would take 10min to set it up, and only requires a small, fixed-temperature incubator. With minor modifications, it could be much more sensitive and easy to run and/or can provide information on presence of specific bacteria.
Keywords: Antibiotic resistance; Antibiotic susceptibility assay; Urinary tract infection.
Copyright Â© 2013 International Society for Chemotherapy of Infection and Cancer. Published by Elsevier Ltd. All rights reserved.
Etiologic profile and antimicrobial susceptibility of community-acquired urinary tract infection in two Cameroonian towns.BMC Res Notes. 2012 May 7;5:219. doi: 10.1186/1756-0500-5-219. BMC Res Notes. 2012. PMID: 22564344 Free PMC article. Clinical Trial.
A biosensor platform for rapid antimicrobial susceptibility testing directly from clinical samples.J Urol. 2011 Jan;185(1):148-53. doi: 10.1016/j.juro.2010.09.022. Epub 2010 Nov 12. J Urol. 2011. PMID: 21074208 Free PMC article. Clinical Trial.
The antibiotic susceptibility patterns of uropathogens among children with urinary tract infection in Shiraz.Medicine (Baltimore). 2017 Sep;96(37):e7834. doi: 10.1097/MD.0000000000007834. Medicine (Baltimore). 2017. PMID: 28906365 Free PMC article.
Phenotypic characteristics of coagulase-negative staphylococci: typing and antibiotic susceptibility.APMIS Suppl. 1999;91:1-42. APMIS Suppl. 1999. PMID: 10230367 Review.
Antimicrobial susceptibility of bacterial isolates from community acquired infections in Sub-Saharan Africa and Asian low and middle income countries.Trop Med Int Health. 2011 Sep;16(9):1167-79. doi: 10.1111/j.1365-3156.2011.02822.x. Epub 2011 Jun 24. Trop Med Int Health. 2011. PMID: 21707879 Free PMC article. Review.