Adipose Stem Cells Display Higher Regenerative Capacities and More Adaptable Electro-Kinetic Properties Compared to Bone Marrow-Derived Mesenchymal Stromal Cells

Sci Rep. 2016 Nov 24;6:37801. doi: 10.1038/srep37801.

Abstract

Adipose stem cells (ASCs) have recently emerged as a more viable source for clinical applications, compared to bone-marrow mesenchymal stromal cells (BM-MSCs) because of their abundance and easy access. In this study we evaluated the regenerative potency of ASCs compared to BM-MSCs. Furthermore, we compared the dielectric and electro-kinetic properties of both types of cells using a novel Dielectrophoresis (DEP) microfluidic platform based on a printed circuit board (PCB) technology. Our data show that ASCs were more effective than BM-MSCs in promoting neovascularization in an animal model of hind-limb ischemia. When compared to BM-MSCs, ASCs displayed higher resistance to hypoxia-induced apoptosis, and to oxidative stress-induced senescence, and showed more potent proangiogenic activity. mRNA expression analysis showed that ASCs had a higher expression of Oct4 and VEGF than BM-MSCs. Furthermore, ASCs showed a remarkably higher telomerase activity. Analysis of the electro-kinetic properties showed that ASCs displayed different traveling wave velocity and rotational speed compared to BM-MSCs. Interestingly, ASCs seem to develop an adaptive response when exposed to repeated electric field stimulation. These data provide new insights into the physiology of ASCs, and evidence to their potential superior potency compared to marrow MSCs as a source of stem cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipocytes / metabolism
  • Adipocytes / physiology*
  • Adipose Tissue / physiology*
  • Animals
  • Apoptosis / physiology
  • Bone Marrow
  • Bone Marrow Cells / metabolism
  • Bone Marrow Cells / physiology
  • Cell Differentiation / physiology
  • Cells, Cultured
  • Hindlimb / metabolism
  • Hindlimb / physiology
  • Hypoxia / metabolism
  • Hypoxia / physiopathology
  • Kinetics
  • Male
  • Mesenchymal Stem Cells / metabolism
  • Mesenchymal Stem Cells / physiology*
  • Oxidative Stress / physiology
  • RNA, Messenger / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Regeneration / physiology*
  • Stem Cells / metabolism
  • Stem Cells / physiology*
  • Telomerase / metabolism
  • Vascular Endothelial Growth Factor A / metabolism

Substances

  • RNA, Messenger
  • Vascular Endothelial Growth Factor A
  • Telomerase