Mouse Models for Campylobacter jejuni Colonization and Infection

Methods Mol Biol. 2017;1512:171-188. doi: 10.1007/978-1-4939-6536-6_15.

Abstract

Relevant animal models for Campylobacter jejuni infection have been difficult to establish due to C. jejuni's inability to cause disease in many common animal research models. Fortunately, recent work has proven successful in developing several new and relevant mouse models of C. jejuni infection, including the SIGIRR-deficient mouse strain that develops acute enterocolitis in response to C. jejuni. Here we describe how to properly infect mice with C. jejuni, as well as a number of accompanying histological techniques to aid in studying C. jejuni colonization and infection in mice.

Keywords: Campylobacter jejuni; Histology; Immunofluorescence; Mouse models; Pathology; Staining.

MeSH terms

  • Acute Disease
  • Age Factors
  • Animals
  • Campylobacter Infections / genetics
  • Campylobacter Infections / microbiology
  • Campylobacter Infections / pathology*
  • Campylobacter jejuni / pathogenicity*
  • Campylobacter jejuni / physiology
  • Colony Count, Microbial
  • Disease Models, Animal
  • Enterocolitis / genetics
  • Enterocolitis / microbiology
  • Enterocolitis / pathology*
  • Female
  • Formaldehyde / chemistry
  • Gene Expression
  • Immunohistochemistry / methods*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Microscopy, Fluorescence
  • Polymers / chemistry
  • Receptors, Interleukin-1 / deficiency
  • Receptors, Interleukin-1 / genetics
  • Sex Factors
  • Staining and Labeling / methods
  • Tissue Fixation

Substances

  • Polymers
  • Receptors, Interleukin-1
  • SIGIRR protein, mouse
  • Formaldehyde
  • paraform