Immunohistochemical insights into Saffold virus infection of the brain of juvenile AG129 mice

Virol J. 2016 Nov 25;13(1):191. doi: 10.1186/s12985-016-0654-8.


Background: Saffold Virus (SAFV) is a human cardiovirus that is suspected of causing infection of the central nervous system (CNS) in children. While recent animal studies have started to elucidate the pathogenesis of SAFV, very little is known about the mechanisms behind it.

Method: In this study, we attempted to elucidate some of the mechanisms of the pathogenesis of SAFV in the brain of a juvenile mouse model by using immunohistochemical methods.

Results: We first showed that SAFV is able to infect both neuronal and glial cells in the brain of 2 week-old AG129 mice. We then showed that SAFV is able to induce apoptosis in both neuronal and glial cells in the brain. Lastly, we showed that SAFV infection does not show any signs of gross demyelination in the brain.

Conclusion: Overall, our results provide important insights into the mechanisms of SAFV in the brain.

Keywords: Apoptosis; Demyelination; Juvenile brain; Saffold virus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis
  • Brain / pathology*
  • Brain / virology*
  • Cardiovirus Infections / pathology*
  • Cardiovirus Infections / virology*
  • Disease Models, Animal
  • Encephalitis, Viral / pathology*
  • Encephalitis, Viral / virology*
  • Immunohistochemistry
  • Mice
  • Neuroglia / virology
  • Neurons / virology
  • Theilovirus / isolation & purification*