Spermatozoal cell death-inducing DNA fragmentation factor-α-like effector A (CIDEA) gene expression and DNA fragmentation in infertile men with metabolic syndrome and normal seminogram

Ayman Z Elsamanoudy; Hussein Abdelaziz Abdalla; Mohammed Hassanien; Mohammad A Gaballah

doi:10.1186/s13098-016-0192-y

Spermatozoal cell death-inducing DNA fragmentation factor-α-like effector A (CIDEA) gene expression and DNA fragmentation in infertile men with metabolic syndrome and normal seminogram

Diabetol Metab Syndr. 2016 Nov 16:8:76. doi: 10.1186/s13098-016-0192-y. eCollection 2016.

Authors

Ayman Z Elsamanoudy¹, Hussein Abdelaziz Abdalla², Mohammed Hassanien³, Mohammad A Gaballah⁴

Affiliations

¹ Department of Clinical Biochemistry, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia ; Department of Medical Biochemistry, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
² Department of Medical Biochemistry, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
³ Department of Clinical Biochemistry, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia ; Department of Medical Biochemistry, Faculty of Medicine, Tanta University, Tanta, Egypt.
⁴ Department of Dermatology, Andrology and STDs, Faculty of Medicine, Mansoura University, Mansoura, Egypt.

Abstract

Background: This is the first study to investigate spermatozoal cell death-inducing DNA fragmentation factor-α-like effector A (CIDEA) gene expression and DNA fragmentations in the spermatozoa of men diagnosed with metabolic syndrome (MS) who have normal seminograms with unexplained infertility, and to correlate these parameters with seminal glucose concentration.

Methods: This study included 120 participants: 75 male subjects with MS (38 fertile and 37 infertile), and a control group of 45 fertile males without MS. HOMA-IR, semen analysis, and biochemical measurement of seminal plasma insulin and glucose levels were carried out. Spermatozoal insulin gene and CIDEA gene expressions were performed by the RT-PCR method. The percentage of spermatozoal DNA fragmentation was also estimated.

Results: The spermatozoal insulin and CIDEA gene expression, as well as the DNA fragmentation, were significantly higher in the infertile MS group than in the fertile MS group, and significantly higher in both the MS groups than in the control group. Seminal glucose concentration showed significant positive correlations with seminal insulin level, spermatozoa insulin, CIDEA gene expression, and DNA fragmentation. Moreover, there was a positive correlation between spermatozoa CIDEA gene expression and DNA fragmentation.

Conclusions: It can be concluded that MS may affect male fertility at the molecular level, through its possible inducing effect of spermatozoa CIDEA and insulin gene expression, DNA fragmentation, and increased seminal glucose.

Keywords: CIDEA; DNA fragmentation; Insulin genes expression; Male infertility; Metabolic syndrome; Seminal glucose.