Evaluation of Hypotensive and Antihypertensive Effects of Velvet Bean (Mucuna pruriens L.) Hydrolysates

J Med Food. 2017 Jan;20(1):37-45. doi: 10.1089/jmf.2016.0080. Epub 2016 Nov 28.


Hypertension could cause significant worldwide health problems that affect 15-20% of all adults; according to National Health and Nutrition Examination Survey, about 29% of the adult population in the United States are hypertensive. Recent research has shown that peptides derived from the hydrolysis of food proteins can decrease blood pressure. This study was carried out to evaluate the hypotensive and antihypertensive potential of Mucuna pruriens protein hydrolysates in in vitro and in vivo models. M. pruriens protein concentrate was prepared by wet fractionation and enzymatically hydrolyzed using Alcalase®, Flavourzyme®, and the sequential system Alcalase-Flavourzyme at different times (5-120 min). The biological potential was measured in vitro based on the IC50 value as well as in vivo effect, measuring the systolic (SBP) and diastolic (DBP) blood pressure in normotensive and antihypertensive Wistar-Kyoto rats by the tail-cuff method. Hydrolysis of M. pruriens protein concentrates with commercial enzymes generated extensive hydrolysates with angiotensin-converting enzyme (ACE-I) inhibitory activity (IC50: 0.589-0.993 mg/mL) and hypotensive (SBP: 0.6-47.43%, DBP: 1.94-43.47%) and antihypertensive (SBP: 8.84-27.29% DBP: 16.1-29.37%) effect. These results indicate that Mucuna pruriens protein hydrolysate (MPPH) could be used as a functional ingredient to prevent blood pressure increase.

Keywords: ACE-I; Mucuna pruriens; antihypertensive; hypotensive; protein hydrolysates.

MeSH terms

  • Angiotensin-Converting Enzyme Inhibitors / administration & dosage*
  • Angiotensin-Converting Enzyme Inhibitors / chemistry
  • Animals
  • Antihypertensive Agents / administration & dosage*
  • Antihypertensive Agents / chemistry
  • Blood Pressure / drug effects
  • Drug Evaluation, Preclinical
  • Humans
  • Hypertension / drug therapy*
  • Hypertension / enzymology
  • Hypertension / physiopathology
  • Mucuna / chemistry*
  • Peptidyl-Dipeptidase A / metabolism
  • Plant Extracts / administration & dosage*
  • Protein Hydrolysates / administration & dosage*
  • Protein Hydrolysates / chemistry
  • Rats
  • Rats, Inbred WKY
  • Seeds / chemistry


  • Angiotensin-Converting Enzyme Inhibitors
  • Antihypertensive Agents
  • Plant Extracts
  • Protein Hydrolysates
  • Peptidyl-Dipeptidase A