Deletion of 3' untranslated region of human BSF-2 mRNA causes stabilization of the mRNA and high-level expression in mouse NIH3T3 cells

N Tonouchi; K Miwa; H Karasuyama; H Matsui

doi:10.1016/0006-291x(89)92328-0

Deletion of 3' untranslated region of human BSF-2 mRNA causes stabilization of the mRNA and high-level expression in mouse NIH3T3 cells

Biochem Biophys Res Commun. 1989 Sep 15;163(2):1056-62. doi: 10.1016/0006-291x(89)92328-0.

Authors

N Tonouchi¹, K Miwa, H Karasuyama, H Matsui

Affiliation

¹ Central Research Laboratories, Ajinomoto Co., Inc., Kawasaki, Japan.

PMID: 2789513
DOI: 10.1016/0006-291x(89)92328-0

Abstract

Most lymphokine genes have an mRNA-destabilizing signal in their 3' untranslated sequences. In this study we demonstrated the usefulness of deletion of this sequence for lymphokine production. By expression of B cell stimulatory factor 2 (BSF-2) cDNAs with and without this region in mouse NIH3T3 cells, it was shown that mRNA of BSF-2 with the 3' destabilizing sequence was very unstable, and that by deletion of this region, the mRNA was stabilized. More than 10-fold BSF-2 productivity was observed in cells harboring the plasmid without the 3' region than in those with it.

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
Cell Line
Chromosome Deletion
Gene Expression Regulation*
Interleukin-6
Interleukins / genetics*
Macrophages / metabolism*
Mice
Molecular Sequence Data
Plasmids
Protein Biosynthesis
RNA, Messenger / genetics*

Substances

Interleukin-6
Interleukins
RNA, Messenger

Associated data

GENBANK/M29150