Biology of tiny animals: three new species of minute salamanders (Plethodontidae: Thorius) from Oaxaca, Mexico

PeerJ. 2016 Nov 15;4:e2694. doi: 10.7717/peerj.2694. eCollection 2016.

Abstract

We describe three new species of minute salamanders, genus Thorius, from the Sierra Madre del Sur of Oaxaca, Mexico. Until now only a single species, T. minutissimus, has been reported from this region, although molecular data have long shown extensive genetic differentiation among geographically disjunct populations. Adult Thorius pinicola sp. nov., T. longicaudus sp. nov., and T. tlaxiacus sp. nov. are larger than T. minutissimus and possess elliptical rather than oval nostrils; T. pinicola and T. longicaudus also have longer tails. All three new species occur west of the range of T. minutissimus, which has the easternmost distribution of any member of the genus. The new species are distinguished from each other and from other named Thorius in Oaxaca by a combination of adult body size, external morphology and osteology, and by protein characters (allozymes) and differences in DNA sequences. In addition, we redescribe T. minutissimus and a related species, T. narisovalis, to further clarify the taxonomic status of Oaxacan populations and to facilitate future studies of the remaining genetically differentiated Thorius that cannot be satisfactorily assigned to any named species. Populations of all five species considered here appear to have declined dramatically over the last one or two decades and live specimens are difficult to find in nature. Thorius may be the most endangered genus of amphibians in the world. All species may go extinct before the end of this century.

Keywords: Amphibia; Biogeography; Cryptic species; Endangered species; Evolution; Miniaturization; Osteology; Systematics.

Grant support

Research was supported by grants from the Programa de Apoyo a Proyectos de Investigación e Innovación Tecnológica (PAPIIT-UNAM) IN209914, Mexico, to GP-O, and from the U.S. National Science Foundation (EF-0334846 to JH, EF-0334939 to D.B.W, and DEB-0613802 to J. Campbell); by the Council on Research and Creative Work, University of Colorado at Boulder, and the Museum of Vertebrate Zoology, the Center for Latin American Studies, and Sigma Xi (Alpha chapter), University of California, Berkeley; and by the Putnam Expeditionary Fund of the Museum of Comparative Zoology and the David Rockefeller Center for Latin American Studies, Harvard University. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.