Structures of human SRP72 complexes provide insights into SRP RNA remodeling and ribosome interaction

Nucleic Acids Res. 2017 Jan 9;45(1):470-481. doi: 10.1093/nar/gkw1124. Epub 2016 Nov 29.


Co-translational protein targeting and membrane protein insertion is a fundamental process and depends on the signal recognition particle (SRP). In mammals, SRP is composed of the SRP RNA crucial for SRP assembly and function and six proteins. The two largest proteins SRP68 and SRP72 form a heterodimer and bind to a regulatory site of the SRP RNA. Despite their essential roles in the SRP pathway, structural information has been available only for the SRP68 RNA-binding domain (RBD). Here we present the crystal structures of the SRP68 protein-binding domain (PBD) in complex with SRP72-PBD and of the SRP72-RBD bound to the SRP S domain (SRP RNA, SRP19 and SRP68) detailing all interactions of SRP72 within SRP. The SRP72-PBD is a tetratricopeptide repeat, which binds an extended linear motif of SRP68 with high affinity. The SRP72-RBD is a flexible peptide crawling along the 5e- and 5f-loops of SRP RNA. A conserved tryptophan inserts into the 5e-loop forming a novel type of RNA kink-turn stabilized by a potassium ion, which we define as K+-turn. In addition, SRP72-RBD remodels the 5f-loop involved in ribosome binding and visualizes SRP RNA plasticity. Docking of the S domain structure into cryo-electron microscopy density maps reveals multiple contact sites between SRP68/72 and the ribosome, and explains the role of SRP72 in the SRP pathway.

MeSH terms

  • Base Sequence
  • Crystallography, X-Ray
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Gene Expression
  • Humans
  • Molecular Docking Simulation
  • Nucleic Acid Conformation
  • Potassium / chemistry
  • Potassium / metabolism
  • Protein Domains
  • Protein Multimerization
  • Protein Structure, Secondary
  • RNA / chemistry*
  • RNA / genetics
  • RNA / metabolism
  • Recombinant Fusion Proteins / chemistry*
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Ribosomes / chemistry*
  • Ribosomes / metabolism
  • Signal Recognition Particle / chemistry*
  • Signal Recognition Particle / genetics
  • Signal Recognition Particle / metabolism
  • Two-Hybrid System Techniques


  • Recombinant Fusion Proteins
  • SRP19 protein, human
  • SRP68 protein, human
  • SRP72 protein, human
  • Signal Recognition Particle
  • RNA
  • Potassium