Temperature-sensitive mutants of mouse hepatitis virus produce a high incidence of demyelination

Proc Natl Acad Sci U S A. 1978 Aug;75(8):4033-6. doi: 10.1073/pnas.75.8.4033.


Mutagenesis of mouse hepatitis virus with 5-azacytidine or 5-fluorouracil yielded several temperature-sensitive mutants. Mutants have been isolated that dramatically enhance the production of demyelinating disease over that previously noted with the wild-type virus. This reproducible model should now make possible the precise elucidation of the pathogenic mechanism and molecular basis of this virus-induced demyelination.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Coronaviridae* / genetics
  • Demyelinating Diseases / etiology*
  • Demyelinating Diseases / pathology
  • Disease Models, Animal*
  • Hepatitis / microbiology*
  • Mice
  • Mice, Inbred BALB C
  • Mutation
  • Spinal Cord / pathology
  • Temperature