Beggiatoa leptomitoformis sp. nov., the first freshwater member of the genus capable of chemolithoautotrophic growth

Int J Syst Evol Microbiol. 2017 Feb;67(2):197-204. doi: 10.1099/ijsem.0.001584. Epub 2017 Mar 16.


A strain of filamentous sulfur bacteria was isolated from freshwater spring contaminated with residential and agricultural wastewater in Moscow region, Russia. According to the results of phylogenetic analysis, strain D-402T belonged to the genus Beggiatoa within the family Beggiatoaceae of the class Gammaproteobacteria. Within the genus Beggiatoa, strain D-402T was most closely related to Beggiatoa alba strains. Strain D-402T had a DNA G+C content 42.1 mol%. The DNA-DNA hybridization value between strain D-402T and Beggiatoa alba strain B15LD was 33 %. Predominant fatty acids were C18 : 1 (46.1 and 53.3 %), C16 : 0 (15.5 and 16.2 %) and C16 : 1 (32.9 and 25.4 %) for strains D-402T and B15LD, respectively. In contrast to known representatives of Beggiatoa, strain D-402T was capable of chemolithoautotrophic growth with sulfide and thiosulfate as electron donors. Oxidation of sulfide and thiosulfate was accompanied by deposition of sulfur globules within the cells. Strain D-402T was capable of heterotrophic growth. The strain was capable of using different organic compounds, sulfur compounds and hydrogen as electron donors. Based on these observations, strain D-402T is considered as a representative of a species Beggiatoa leptomitoformis sp. nov. of the genus Beggiatoa. The type strain is D-402T (=DSM 14946T=UNIQEM U 779T).

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • Beggiatoa / classification*
  • Beggiatoa / genetics
  • Beggiatoa / isolation & purification
  • Chemoautotrophic Growth*
  • DNA, Bacterial / genetics
  • Fatty Acids / chemistry
  • Fresh Water / microbiology*
  • Nucleic Acid Hybridization
  • Oxidation-Reduction
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Russia
  • Sequence Analysis, DNA
  • Sulfides / chemistry


  • DNA, Bacterial
  • Fatty Acids
  • RNA, Ribosomal, 16S
  • Sulfides