Structure of the 70S ribosome from human pathogen Staphylococcus aureus

Nucleic Acids Res. 2016 Dec 1;44(21):10491-10504. doi: 10.1093/nar/gkw933. Epub 2016 Oct 18.

Abstract

Comparative structural studies of ribosomes from various organisms keep offering exciting insights on how species-specific or environment-related structural features of ribosomes may impact translation specificity and its regulation. Although the importance of such features may be less obvious within more closely related organisms, their existence could account for vital yet species-specific mechanisms of translation regulation that would involve stalling, cell survival and antibiotic resistance. Here, we present the first full 70S ribosome structure from Staphylococcus aureus, a Gram-positive pathogenic bacterium, solved by cryo-electron microscopy. Comparative analysis with other known bacterial ribosomes pinpoints several unique features specific to S. aureus around a conserved core, at both the protein and the RNA levels. Our work provides the structural basis for the many studies aiming at understanding translation regulation in S. aureus and for designing drugs against this often multi-resistant pathogen.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacillus subtilis / chemistry
  • Bacillus subtilis / genetics
  • Bacillus subtilis / metabolism
  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Binding Sites
  • Cryoelectron Microscopy
  • Escherichia coli / chemistry
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Gene Expression
  • Molecular Dynamics Simulation
  • Nucleic Acid Conformation
  • Protein Binding
  • Protein Biosynthesis*
  • Protein Conformation, alpha-Helical
  • Protein Conformation, beta-Strand
  • Protein Interaction Domains and Motifs
  • RNA, Bacterial / chemistry*
  • RNA, Bacterial / metabolism
  • Ribosomal Proteins / chemistry*
  • Ribosomal Proteins / genetics
  • Ribosomal Proteins / metabolism
  • Ribosomes / chemistry
  • Ribosomes / metabolism
  • Ribosomes / ultrastructure*
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Species Specificity
  • Staphylococcus aureus / chemistry*
  • Staphylococcus aureus / genetics
  • Staphylococcus aureus / metabolism
  • Thermus thermophilus / chemistry
  • Thermus thermophilus / genetics
  • Thermus thermophilus / metabolism

Substances

  • Bacterial Proteins
  • RNA, Bacterial
  • Ribosomal Proteins