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. 2017;1541:59-66.
doi: 10.1007/978-1-4939-6703-2_6.

Chromosome Bandings

Affiliations

Chromosome Bandings

Huifang Huang et al. Methods Mol Biol. .

Abstract

Chromosome banding is an essential technique used in chromosome karyotyping to identify normal and abnormal chromosomes for clinical and research purposes. Giemsa (G)-, reverse (R)-, and centromere (C)-banding are the most commonly dye-based chromosome-banding techniques. G-banding involves the staining of trypsin-treated chromosomes and R-banding involves denaturing in hot acidic saline followed by Giemsa staining. C-banding is specifically used for identifying heterochromatin by denaturing chromosomes in a saturated alkaline solution followed by Giemsa staining. Different banding techniques may be selected for the identification of chromosomes.

Keywords: C-banding; Chromosome banding; G-banding; Karyotyping; R-banding.

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