Multicolor Karyotyping and Fluorescence In Situ Hybridization-Banding (MCB/mBAND)

Thomas Liehr; Moneeb A K Othman; Katharina Rittscher

doi:10.1007/978-1-4939-6703-2_16

Multicolor Karyotyping and Fluorescence In Situ Hybridization-Banding (MCB/mBAND)

Methods Mol Biol. 2017:1541:181-187. doi: 10.1007/978-1-4939-6703-2_16.

Authors

Thomas Liehr¹, Moneeb A K Othman², Katharina Rittscher²

Affiliations

¹ Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Kollegiengasse 10, D-07743, Jena, Germany. Thomas.Liehr@med.uni-jena.de.
² Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Kollegiengasse 10, D-07743, Jena, Germany.

PMID: 27910024
DOI: 10.1007/978-1-4939-6703-2_16

Abstract

Multicolor fluorescence in situ hybridization (mFISH) approaches are routine applications in tumor as well as clinical cytogenetics nowadays. The first approach when thinking about mFISH is multicolor karyotyping using human whole chromosome paints as probes; this can be achieved by narrow-band filter-based multiplex-FISH (M-FISH) or interferometer/spectroscopy-based spectral karyotyping (SKY). Besides, various FISH-based banding approaches were reported in the literature, including multicolor banding (MCB/mBAND) the latter being evaluated by narrow-band filters, and using specific software. Here, we describe the combined application of multicolor karyotyping and MCB/mBAND for the characterization of simple and complex acquired chromosomal changes in cancer cytogenetics.

Keywords: Multicolor banding (MCB/mBAND); Multicolor fluorescence in situ hybridization (mFISH); Multicolor karyotyping; Multiplex-FISH (M-FISH); Spectral karyotyping (SKY).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromosome Aberrations
Chromosome Banding
Chromosome Painting
Computational Biology / methods
DNA Probes
Humans
Image Processing, Computer-Assisted
In Situ Hybridization, Fluorescence / methods*
Spectral Karyotyping / methods*

Substances

DNA Probes