Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections

J Vis Exp. 2016 Nov 14:(117):50425. doi: 10.3791/50425.


Immunohistochemistry (IHC) provides highly specific, reliable and attractive protein visualization. Correct performance and interpretation of an IHC-based multicolor labeling is challenging, especially when utilized for assessing interrelations between target proteins in the tissue with a high fat content such as the central nervous system (CNS). Our protocol represents a refinement of the standard immunolabeling technique particularly adjusted for detection of both structural and soluble proteins in the rat CNS and peripheral lymph nodes (LN) affected by neuroinflammation. Nonetheless, with or without further modifications, our protocol could likely be used for detection of other related protein targets, even in other organs and species than here presented.

Publication types

  • Video-Audio Media

MeSH terms

  • Animals
  • Central Nervous System*
  • Histocytological Preparation Techniques
  • Immunohistochemistry*
  • Lymph Nodes*
  • Rats