Effects of high-fat diet on plasma profiles of eicosanoid metabolites in mice

Prostaglandins Other Lipid Mediat. 2016 Dec:127:9-13. doi: 10.1016/j.prostaglandins.2016.11.003. Epub 2016 Nov 30.

Abstract

Obesity is a serious health problem in the US and is associated with increased risks of various human diseases. To date, the mechanisms by which obesity increases the risks of a wide range of human diseases are not well understood. Here we used a LC-MS/MS-based lipidomics, which can analyze >100 bioactive lipid mediators produced by cyclooxygenase, lipoxygenase, and cytochrome P450 enzymes, to analyze plasma profiles of lipid mediators in high-fat diet induced obesity in C57BL/6 mice. Our results show that the plasma concentrations of epoxyoctadecenoic acids (EpOMEs, also termed as leukotoxins) are significantly increased in plasma of high-fat diet-fed mice, in addition, EpOMEs are among the most abundant lipid mediators detected in mouse plasma. Since substantial studies have shown that EpOMEs and their metabolites have a large array of detrimental effects on health, enhanced levels of EpOMEs could contribute to the pathology of obesity.

Keywords: Eicosanoids; Epoxyoctadecenoic acids; High-fat diet; Leukotoxins; Obesity.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Diet, High-Fat / adverse effects*
  • Eicosanoids / blood*
  • Eicosanoids / metabolism*
  • Enzymes / metabolism
  • Lipid Peroxidation / drug effects
  • Male
  • Metabolomics
  • Mice
  • Mice, Inbred C57BL

Substances

  • Eicosanoids
  • Enzymes