Antioxidant and hemolytic activities, and effects in rat cutaneous wound healing of a novel polysaccharide from fenugreek (Trigonella foenum-graecum) seeds

Int J Biol Macromol. 2017 Feb:95:625-634. doi: 10.1016/j.ijbiomac.2016.11.091. Epub 2016 Nov 30.


The aim of this work was to evaluate the antioxidant and hemolytic activities as well as the in vivo wound healing performance of a novel polysaccharide (FWEP) extracted from fenugreek (Trigonella foenum-graecum) seeds. The antioxidant activity was evaluated in vivo and in vitro using various assays. Results showed that FWEP exhibited strong antioxidant activities but no hemolytic activity was observed towards bovine erythrocytes. The application of FWEP hydrogel on the wound site in a rat model enhanced significantly wound healing activity and accelerated the wound closure after 14days of wound induction. Histological examination also demonstrated fully re-epithelialized wound with a complete epidermal regeneration. Altogether, these evidences demonstrated that FWEP had strong wound healing potential presumably achieved through its antioxidant activities.

Keywords: Antioxidant activities; Fenugreek seeds; Hemolytic activity; Polysaccharide; Wound healing.

MeSH terms

  • Animals
  • Antioxidants / chemistry
  • Antioxidants / pharmacology*
  • Biomarkers / metabolism
  • Biphenyl Compounds / chemistry
  • Bleeding Time
  • Body Weight / drug effects
  • Hemolysis / drug effects*
  • Hydrogen Peroxide / metabolism
  • Hydroxyproline / metabolism
  • Iron / chemistry
  • Male
  • Oxidation-Reduction / drug effects
  • Oxidative Stress / drug effects
  • Picrates / chemistry
  • Polysaccharides / chemistry
  • Polysaccharides / pharmacology*
  • Rats
  • Rats, Wistar
  • Seeds / chemistry*
  • Skin / drug effects*
  • Skin / metabolism
  • Skin Physiological Phenomena / drug effects
  • Trigonella / chemistry*
  • Wound Healing / drug effects*
  • beta Carotene / metabolism


  • Antioxidants
  • Biomarkers
  • Biphenyl Compounds
  • Picrates
  • Polysaccharides
  • beta Carotene
  • Hydrogen Peroxide
  • 1,1-diphenyl-2-picrylhydrazyl
  • Iron
  • Hydroxyproline