A Single Intravesical Instillation of VAX014 Inhibits Orthotopic Superficial Bladder Tumor Implantation to Increase Survival

Anticancer Res. 2016 Dec;36(12):6243-6248. doi: 10.21873/anticanres.11218.

Abstract

Background/aim: VAX014 minicells (VAX014) have been previously characterized as an integrin-specific oncolytic biotherapeutic agent. The present study was designed to evaluate the potential of VAX014 as an immediate post-operative intravesical adjuvant therapy in the treatment of non-muscle invasive bladder cancer (NMIBC).

Materials and methods: The ability of VAX014 to kill a panel of dissociated urothelial carcinoma cell lines was tested in vitro. In vivo experiments were conducted using a single intravesical dose of VAX014 in the anti-implantation variation of the MB49 syngeneic orthotopic bladder cancer model with tumor implantation and overall survival rates serving as study endpoints.

Results: VAX014 rapidly killed dissociated urothelial carcinoma cells, while single dose in vivo pharmacology studies demonstrated the dose-dependent ability of VAX014 to prevent tumor implantation and development, ultimately resulting in a significant survival advantage compared to controls.

Conclusion: These results suggest that VAX014 holds potential as an immediate post-operative adjuvant therapy in NMIBC.

Keywords: Non-muscle invasive bladder cancer; invasin; minicells; perfringolysin O.

MeSH terms

  • Animals
  • Cancer Vaccines / administration & dosage*
  • Cell Line, Tumor
  • Humans
  • Mice
  • Survival Rate
  • Urinary Bladder Neoplasms / drug therapy*

Substances

  • Cancer Vaccines