Lipoprotein profiling methodology based on determination of apolipoprotein concentration

Bioanalysis. 2017 Jan;9(1):9-19. doi: 10.4155/bio-2016-0234.


Aim: Abnormal lipid metabolism results in the alteration of lipid compositions in lipoproteins; therefore an accurate and quantitative analytical approach is required for the detailed structural characterization of lipoproteins. However, the specific lipid composition of each lipoprotein particle is poorly understood.

Materials & methods: Lipid composition of very-low-density lipoprotein and low-density lipoprotein particles derived from myocardial infarction-prone rabbits was determined by normalization of lipidomics data using apoB-100 levels.

Results: The ratio of lipid levels between very-low-density lipoprotein and low-density lipoprotein particles was different according to not only lipid classes, but also phosphatidylethanolamine subclasses by applying our developed methodology to myocardial infarction-prone rabbits.

Conclusion: Our novel analytical approach represents to be a potentially useful tool to obtain particle-specific lipid components of lipoproteins.

Keywords: apolipoprotein; lipoprotein; targeted proteomics.

MeSH terms

  • Animals
  • Apolipoprotein B-100 / analysis
  • Apolipoprotein B-100 / blood
  • Apolipoproteins / analysis*
  • Apolipoproteins / blood
  • Chromatography, Liquid / methods
  • Female
  • Lipoproteins / analysis*
  • Lipoproteins / blood
  • Lipoproteins, LDL / analysis
  • Lipoproteins, LDL / blood
  • Lipoproteins, VLDL / analysis
  • Lipoproteins, VLDL / blood
  • Male
  • Myocardial Infarction / blood
  • Myocardial Infarction / pathology
  • Rabbits
  • Tandem Mass Spectrometry / methods*


  • Apolipoprotein B-100
  • Apolipoproteins
  • Lipoproteins
  • Lipoproteins, LDL
  • Lipoproteins, VLDL