Identification of KIAA1199 as a Biomarker for Pancreatic Intraepithelial Neoplasia

Sci Rep. 2016 Dec 6;6:38273. doi: 10.1038/srep38273.

Abstract

Pancreatic cancer is one of the most aggressive cancers and has an extremely poor prognosis. Despite recent progress in both basic and clinical research, most pancreatic cancers are detected at an incurable stage owing to the absence of disease-specific symptoms. Thus, developing novel approaches for detecting pancreatic cancer at an early stage is imperative. Our in silico and immunohistochemical analyses showed that KIAA1199 is specifically expressed in human pancreatic cancer cells and pancreatic intraepithelial neoplasia, the early lesion of pancreatic cancer, in a genetically engineered mouse model and in human patient samples. We also detected secreted KIAA1199 protein in blood samples obtained from pancreatic cancer mouse models, but not in normal mice. Furthermore, we found that assessing KIAA1199 autoantibody increased the sensitivity of detecting pancreatic cancer. These results indicate the potential benefits of using KIAA1199 as a biomarker for early-stage pancreatic cancer.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Acute Disease
  • Animals
  • Autoantibodies / biosynthesis
  • Autoantibodies / blood*
  • Biomarkers, Tumor / blood
  • Biomarkers, Tumor / genetics*
  • Carcinoma, Pancreatic Ductal / diagnosis
  • Carcinoma, Pancreatic Ductal / genetics*
  • Carcinoma, Pancreatic Ductal / immunology
  • Carcinoma, Pancreatic Ductal / pathology
  • Cell Line, Tumor
  • Ceruletide
  • Databases, Genetic
  • Disease Models, Animal
  • Early Diagnosis
  • Gene Expression Profiling
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Hyaluronoglucosaminidase
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Pancreatic Neoplasms / diagnosis
  • Pancreatic Neoplasms / genetics*
  • Pancreatic Neoplasms / immunology
  • Pancreatic Neoplasms / pathology
  • Pancreatitis / chemically induced
  • Pancreatitis / genetics*
  • Pancreatitis / immunology
  • Pancreatitis / pathology
  • Proteins / genetics*
  • Proteins / metabolism
  • Tissue Array Analysis

Substances

  • Autoantibodies
  • Biomarkers, Tumor
  • Proteins
  • Ceruletide
  • CEMIP protein, human
  • Hyaluronoglucosaminidase