Durability of Three Different Types of Hyaluronic Acid Fillers in Skin: Are There Differences Among Biphasic, Monophasic Monodensified, and Monophasic Polydensified Products?

Aesthet Surg J. 2017 May 1;37(5):573-581. doi: 10.1093/asj/sjw161.


Background: Hyaluronic acid fillers are used for facial rejuvenation and are classified as non-cross-linked or cross-linked (monophasic mono- or polydensified).

Objectives: To histologically assess the intradermal durability of three types of fillers (biphasic, monophasic monodensified, and monophasic polydensified), to compare the durability of the products over 6 months, and to evaluate the structural changes after application.

Methods: In all, 25 volunteers received injections of three different fillers in the dermis of the right lumbar region (in one line), and equal amounts of the fillers were injected into three different sites (in the same column), yielding nine points of application in each patient. Each line was biopsied on days 2, 92, and 184; these skin samples were analyzed histologically, and the presence or absence of these fillers was verified by a dermatopathologist.

Results: The histological analysis showed that over 182 days, the amount of the injected monophasic polydensified, monophasic monodensified, and biphasic filler products decreased by 62.5%, 25%, and 12.5%, respectively.

Conclusions: The biphasic and monophasic monodensified fillers presented greater intradermal durability than did the monophasic polydensified filler at 6 months after intradermal injection.

Publication types

  • Clinical Trial, Phase IV
  • Comparative Study
  • Observational Study

MeSH terms

  • Biopsy
  • Cosmetic Techniques*
  • Dermal Fillers / administration & dosage
  • Dermal Fillers / chemistry*
  • Elastic Modulus
  • Female
  • Humans
  • Hyaluronic Acid / administration & dosage
  • Hyaluronic Acid / chemistry*
  • Injections, Intradermal
  • Middle Aged
  • Prospective Studies
  • Rejuvenation*
  • Skin / anatomy & histology
  • Viscosity


  • Dermal Fillers
  • Hyaluronic Acid