Serum Amyloid A3 Gene Expression in Adipocytes is an Indicator of the Interaction with Macrophages

Sci Rep. 2016 Dec 8;6:38697. doi: 10.1038/srep38697.

Abstract

The infiltration of macrophages into adipose tissue and their interaction with adipocytes are essential for the chronic low-grade inflammation of obese adipose tissue. In this study, we identified the serum amyloid A3 (Saa3) gene as a key adipocyte-derived factor that is affected by interaction with macrophages. We showed that the Saa3 promoter in adipocytes actually responds to activated macrophages in a co-culture system. Decreasing C/EBPβ abundance in 3T3-L1 adipocytes or point mutation of C/EBPβ elements suppressed the increased promoter activity in response to activated macrophages, suggesting an essential role of C/EBPβ in Saa3 promoter activation. Bioluminescence based on Saa3 promoter activity in Saa3-luc mice was promoted in obese adipose tissue, showing that Saa3 promoter activity is most likely related to macrophage infiltration. This study suggests that the level of expression of the Saa3 gene could be utilized for the number of infiltrated macrophages in obese adipose tissue.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3-L1 Cells
  • Adipocytes / metabolism*
  • Animals
  • Biomarkers
  • CCAAT-Enhancer-Binding Protein-beta / metabolism
  • Cell Communication*
  • Gene Expression Profiling
  • Gene Expression*
  • HEK293 Cells
  • Humans
  • Macrophage Activation / genetics
  • Macrophage Activation / immunology
  • Macrophages / immunology
  • Macrophages / metabolism*
  • Macrophages / pathology
  • Male
  • Mice
  • Models, Biological
  • Molecular Imaging
  • Promoter Regions, Genetic
  • RAW 264.7 Cells
  • Serum Amyloid A Protein / genetics*
  • Transcriptome

Substances

  • Biomarkers
  • CCAAT-Enhancer-Binding Protein-beta
  • Saa3 protein, mouse
  • Serum Amyloid A Protein