Triolein reduces MMP-1 upregulation in dermal fibroblasts generated by ROS production in UVB-irradiated keratinocytes

J Dermatol Sci. 2017 Feb;85(2):124-130. doi: 10.1016/j.jdermsci.2016.11.010. Epub 2016 Nov 27.


Background: Cytokine production and oxidative stress generated by ultraviolet radiation B (UVB) skin exposure are main factors of skin photoaging. Interleukin-6 (IL-6) produced by irradiated keratinocytes is proposed to have a role in metalloproteinases (MMPs) expression activation in dermal fibroblasts.

Objectives: We examined the effect of triolein treatment of UVB-irradiated keratinocytes on MMP1 (interstitial collagenase) expression response of dermal fibroblasts. We assayed UVB-irradiated keratinocytes soluble signals, mainly IL-6 and reactive oxygen species (ROS).

Methods: IL-6 expression and ROS generation were assayed in UVB-irradiated keratinocytes. MMP1 mRNA expression response was assayed in fibroblasts grown in keratinocytes conditioned medium. We evaluated the effect of treating keratinocytes with triolein on IL-6 expression and ROS generation in keratinocytes, and MMP1 expression in fibroblasts.

Results: The irradiation of epidermal cells with sublethal UVB doses increased IL-6 expression and ROS generation. Conditioned culture medium collected from keratinocytes was used to culture dermal fibroblasts. MMP1 mRNA expression increase was observed in fibroblasts cultured in medium collected from UVB-irradiated keratinocytes. Triolein treatment reduced the IL-6 expression and ROS generation in keratinocytes and this effect was reflected in downregulation of MMP1 expression in fibroblasts.

Conclusions: Triolein reduces both the expression of IL-6 and ROS generation in irradiated keratinocytes. It seems to exert an anti-inflammatory and anti-oxidative stress effect on irradiated keratinocytes that in turn reduces MMP1 expression in dermal fibroblasts. Collectively, these results indicate that triolein could act as a photoprotective agent.

Keywords: MMP1; ROS; Triolein IL-6; UVB.

MeSH terms

  • Antioxidants / pharmacology
  • Cell Line
  • Culture Media, Conditioned
  • Dermis / cytology
  • Dermis / drug effects
  • Dermis / enzymology
  • Dermis / radiation effects
  • Down-Regulation
  • Fibroblasts / drug effects
  • Fibroblasts / enzymology
  • Humans
  • Interleukin-6 / metabolism
  • Keratinocytes / drug effects*
  • Keratinocytes / enzymology
  • Keratinocytes / radiation effects
  • Matrix Metalloproteinase 1 / metabolism*
  • Oxidative Stress / drug effects
  • RNA, Messenger / metabolism
  • Reactive Oxygen Species / metabolism*
  • Skin Aging / drug effects
  • Skin Aging / radiation effects
  • Sunscreening Agents / pharmacology*
  • Triolein / pharmacology*
  • Ultraviolet Rays / adverse effects
  • Up-Regulation


  • Antioxidants
  • Culture Media, Conditioned
  • IL6 protein, human
  • Interleukin-6
  • RNA, Messenger
  • Reactive Oxygen Species
  • Sunscreening Agents
  • Triolein
  • MMP1 protein, human
  • Matrix Metalloproteinase 1