Mannich bases of 1,2,4-triazole-3-thione containing adamantane moiety: Synthesis, preliminary anticancer evaluation, and molecular modeling studies

Chem Biol Drug Des. 2017 Jun;89(6):943-952. doi: 10.1111/cbdd.12920. Epub 2017 Jan 25.


A series of 18 novel N-Mannich bases derived from 5-adamantyl-1,2,4-triazole-3-thione was synthesized and characterized using NMR spectroscopy and X-ray diffraction technique. All derivatives were evaluated for their anticancer potential against four human cancer cell lines. Several tested compounds exerted good cytotoxic activities on K562 and HL-60 cell lines, along with pronounced selectivity, showing lower cytotoxicity against normal fibroblasts MRC-5 compared to cancer cells. The effects of compounds 5b, 5e, and 5j on the cell cycle were investigated by flow cytometric analysis. It was found that these compounds cause the accumulation of cells in the subG1 and G1 phases of the cell cycle and induce caspase-dependent apoptosis, while the anti-angiogenic effects of 5b, 5e, and 5j have been confirmed in EA.hy926 cells using a tube formation assay. Further, the interaction of Bax protein with compound 5b was investigated by means of molecular modeling, applying the combined molecular docking/molecular dynamics approach.

Keywords: Mannich bases; adamantane; anticancer activity; molecular modeling; triazoles.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adamantane / chemistry
  • Adamantane / pharmacology
  • Antineoplastic Agents / chemical synthesis*
  • Antineoplastic Agents / chemistry
  • Antineoplastic Agents / pharmacology*
  • Apoptosis / drug effects
  • Cell Cycle / drug effects
  • Cell Line, Tumor
  • Cell Survival / drug effects
  • Fibroblasts / drug effects
  • Humans
  • Inhibitory Concentration 50
  • Mannich Bases / chemistry
  • Mannich Bases / pharmacology
  • Molecular Docking Simulation*
  • Molecular Structure
  • Thiones / chemical synthesis
  • Thiones / chemistry*
  • Thiones / pharmacology*
  • Triazoles / chemistry
  • Triazoles / pharmacology


  • Antineoplastic Agents
  • Mannich Bases
  • Thiones
  • Triazoles
  • 1,2,4-triazole
  • Adamantane