Abstract
Adipic acid diselenoester was developed as an efficient cross-linker for covalent protein conjugation with a variety of small molecular haptens, including mono- and disaccharides, peptide, fluorescence dye, and nicotine. Compared to the counterparts of N-hydroxysuccinimide (NHS) and p-nitrophenyl (PNP) linkers, the diselenoester linker demonstrates improved balance between reactivity and stability and coupling of haptens to proteins under mild conditions with high incorporation efficiency.
Publication types
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Historical Article
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Research Support, Non-U.S. Gov't
MeSH terms
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Adipates / chemical synthesis
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Adipates / chemistry*
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Cross-Linking Reagents / chemical synthesis
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Cross-Linking Reagents / chemistry*
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Haptens / chemistry*
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History, 20th Century
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Organoselenium Compounds / chemical synthesis
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Organoselenium Compounds / chemistry*
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Ovalbumin / chemistry*
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Serum Albumin, Bovine / chemistry*
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Vaccines, Synthetic / chemistry
Substances
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Adipates
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Cross-Linking Reagents
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Haptens
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Organoselenium Compounds
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Vaccines, Synthetic
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Serum Albumin, Bovine
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adipic acid
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Ovalbumin