Quantitative Analysis of Exosomal miRNA via qPCR and Digital PCR

Methods Mol Biol. 2017;1545:55-70. doi: 10.1007/978-1-4939-6728-5_5.

Abstract

Extracellular vesicles, such as exosomes and microvesicles, have been shown to contain potential microRNA (miRNA) biomarkers that may be utilized in the diagnosis of various diseases from cancer to neurological disorders. The unique nature of the extracellular vesicle bilayer allows miRNA to be protected from degradation making it an ideal source of material for biomarkers discovery from both fresh and archived samples. Here we describe the quantitative analysis of miRNA isolated from exosomes by quantitative PCR and digital PCR.

Keywords: Biomarker; Digital PCR; Exosomes; Quantitative PCR; RNA isolation; Taqman assay; miRNA.

MeSH terms

  • Biomarkers
  • Computational Biology / methods
  • Exosomes / metabolism*
  • Gene Expression
  • MicroRNAs / genetics*
  • MicroRNAs / isolation & purification
  • MicroRNAs / metabolism*
  • RNA Transport
  • Real-Time Polymerase Chain Reaction*

Substances

  • Biomarkers
  • MicroRNAs