Prolonged Cardiopulmonary Bypass is a Risk Factor for Intestinal Ischaemic Damage and Endotoxaemia

Heart Lung Circ. 2017 Jul;26(7):717-723. doi: 10.1016/j.hlc.2016.10.012. Epub 2016 Nov 24.

Abstract

Background: Intestinal ischaemia-reperfusion, a frequent occurrence during cardiac surgery with cardiopulmonary bypass (CPB) induces a systemic inflammatory reaction. We hypothesised that ischaemia-reperfusion following prolonged CPB could increase intestinal permeability and thus, lead to endotoxin translocation from the intestine to the bloodstream.

Material and methods: Patients subjected to coronary artery bypass grafting with CPB were included: Group 1 (CPB ≥90minutes) or Group 2 (CPB <90minutes). Intestinal Fatty Acid Binding Protein (I-FABP), TNF alpha, IL6, IL8, and endotoxin levels were measured before the induction of general anaesthesia (T1), at 6 (T2), and 24hours (T3) after surgery.

Results: The low level of I-FABP at T1 increased for every patient in Group 1 at T2 (from 1015.5pg/mL to 2608.5pg/mL, p=0.02) and in Group 2 (from 1123.5pg/ml to 2284.0pg/ml, p<0.001). Furthermore, at T3, the I-FABP level was over three times higher in Group 1 than in Group 2 (2178pg/mL vs 615pg/mL; p<0.001). I-FABP correlated with CPB time (R=0.6, p<0.001) at T3. After surgery, endotoxins were elevated in 73% of patients in Group 1 and in 32% in Group 2 and correlated with CPB time (at T2, R=0.5, p=0.002; at T3, R=0.4, p=0.016).

Conclusions: The duration of CPB is linked to the release of biomarkers that indicate ischaemic-reperfusion damage to the gastrointestinal mucosa and endotoxaemia. I-FABP assay may help to identify patients presenting with intestinal damage, who are at risk of bacterial translocation.

Keywords: Cardiopulmonary bypass; Endotoxin; Intestinal fatty acid binding protein; Intestinal ischaemia-reperfusion.

Publication types

  • Clinical Trial

MeSH terms

  • Aged
  • Biomarkers / blood
  • Cardiopulmonary Bypass / adverse effects*
  • Cytokines / blood*
  • Endotoxemia / blood*
  • Endotoxemia / etiology
  • Fatty Acid-Binding Proteins / blood*
  • Female
  • Humans
  • Intestinal Diseases / blood*
  • Intestinal Diseases / etiology
  • Intestinal Mucosa / injuries
  • Male
  • Middle Aged
  • Postoperative Complications / blood*
  • Reperfusion Injury / blood*
  • Reperfusion Injury / etiology
  • Time Factors

Substances

  • Biomarkers
  • Cytokines
  • Fatty Acid-Binding Proteins