Defects in CD4+ T cell LFA-1 integrin-dependent adhesion and proliferation protect Cd47-/- mice from EAE

Abstract

CD47 is known to play an important role in CD4⁺ T cell homeostasis. We recently reported a reduction in mice deficient in the Cd47 gene (Cd47^-/-) CD4⁺ T cell adhesion and transendothelial migration (TEM) in vivo and in vitro as a result of impaired expression of high-affinity forms of LFA-1 and VLA-4 integrins. A prior study concluded that Cd47^-/- mice were resistant to experimental autoimmune encephalomyelitis (EAE) as a result of complete failure in CD4⁺ T cell activation after myelin oligodendrocyte glycoprotein peptide 35-55 aa (MOG_35-55) immunization. As the prior EAE study was published before our report, authors could not have accounted for defects in T cell integrin function as a mechanism to protect Cd47^-/- in EAE. Thus, we hypothesized that failure of T cell activation involved defects in LFA-1 and VLA-4 integrins. We confirmed that Cd47^-/- mice were resistant to MOG_35-55-induced EAE. Our data, however, supported a different mechanism that was not a result of failure of CD4⁺ T cell activation. Instead, we found that CD4⁺ T cells in MOG_35-55-immunized Cd47^-/- mice were activated, but clonal expansion contracted within 72 h after immunization. We used TCR crosslinking and mitogen activation in vitro to investigate the underlying mechanism. We found that naïve Cd47^-/- CD4⁺ T cells exhibited a premature block in proliferation and survival because of impaired activation of LFA-1, despite effective TCR-induced activation. These results identify CD47 as an important regulator of LFA-1 and VLA-4 integrin-adhesive functions in T cell proliferation, as well as recruitment, and clarify the roles played by CD47 in MOG_35-55-induced EAE.

Keywords: T lymphocytes; TCR activation; autoimmune; neuroinflammation.