Cloning and analysis of the gene encoding the 230-kilodalton merozoite surface antigen of Plasmodium yoelii

Mol Biochem Parasitol. 1989 Oct;36(3):271-82. doi: 10.1016/0166-6851(89)90175-8.

Abstract

The complete nucleotide sequence of the gene for the 230-kDa precursor to the major merozoite surface antigens (PMMSA) of Plasmodium yoelii YM has been determined. A single open reading frame of 5316 bp encodes a protein of calculated molecular mass 197,000. The deduced amino acid sequence contains potential signal peptide and membrane anchor sequences of 19 and 18 residues, respectively, and a region of six tandemly repeated tetrapeptides, Gly-Ala-Val-Pro. Comparison with the 195-kDa Plasmodium falciparum analogue (Pf195) at the amino acid level indicated an overall homology of approximately 30%, with areas of as high as 60% conservation. The tripeptide repeats present near the N-terminus of the Pf195 antigen are absent from the Py230 sequence. The PMMSA can be divided into 22 blocks based upon interspecies amino acid conservation.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, Protozoan / genetics*
  • Antigens, Surface / genetics*
  • Base Sequence
  • Cloning, Molecular*
  • Mice
  • Molecular Sequence Data
  • Molecular Weight
  • Plasmodium yoelii / genetics*
  • Plasmodium yoelii / immunology
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid

Substances

  • Antigens, Protozoan
  • Antigens, Surface

Associated data

  • GENBANK/J04668