Tau Oligomers as Pathogenic Seeds: Preparation and Propagation In Vitro and In Vivo

Julia E Gerson; Urmi Sengupta; Rakez Kayed

doi:10.1007/978-1-4939-6598-4_9

Tau Oligomers as Pathogenic Seeds: Preparation and Propagation In Vitro and In Vivo

Methods Mol Biol. 2017:1523:141-157. doi: 10.1007/978-1-4939-6598-4_9.

Authors

Julia E Gerson^{1

2}, Urmi Sengupta^{1

2}, Rakez Kayed^{3

4

5}

Affiliations

¹ George P. and Cynthia Woods Mitchell Center for Neurodegenerative Diseases, University of Texas Medical Branch, Galveston, TX, 77555, USA.
² Departments of Neurology, and Neuroscience and Cell Biology, University of Texas Medical Branch, Galveston, TX, 77555-1045, USA.
³ George P. and Cynthia Woods Mitchell Center for Neurodegenerative Diseases, University of Texas Medical Branch, Galveston, TX, 77555, USA. rakayed@utmb.edu.
⁴ Departments of Neurology, and Neuroscience and Cell Biology, University of Texas Medical Branch, Galveston, TX, 77555, USA. rakayed@utmb.edu.
⁵ University of Texas Medical Branch, 301 University Boulevard, Medical Research Building, Room 10.138C, Galveston, TX, 77555-1045, USA. rakayed@utmb.edu.

PMID: 27975249
DOI: 10.1007/978-1-4939-6598-4_9

Abstract

Tau oligomers have been shown to be the main toxic tau species in a number of neurodegenerative disorders. In order to study tau oligomers both in vitro and in vivo, we have established methods for the reliable preparation, isolation, and detection of tau oligomers. Methods for the seeding of tau oligomers, isolation of tau oligomers from tissue, and detection of tau oligomers using tau oligomer-specific antibodies by biochemical and immunohistochemical methods are detailed below.

Keywords: Amyloid seeding; Tau oligomer isolation; Tau oligomers; Tauopathies.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amyloid / metabolism
Animals
Humans
Immunohistochemistry
Tauopathies / metabolism*
tau Proteins / chemistry*
tau Proteins / isolation & purification*

Substances

Amyloid
tau Proteins