Proteotypic Peptides and Their Applications

Methods Mol Biol. 2017:1549:101-107. doi: 10.1007/978-1-4939-6740-7_8.


Recent advances in mass spectrometry based proteomic techniques and publicly available large proteomic repositories are being exploited to characterize the proteome of multiple organisms. While humongous amount of proteomic data is being acquired and analyzed, many biological questions still remain unanswered. Proteotypic peptides which uniquely represent target proteins or a protein isoform are used as an alternative strategy for protein identification in the field of immunological methods and targeted proteomic techniques. Using different computational approaches, resources and techniques used in the identification of proteotypic peptides of target proteins is discussed here.

Keywords: Bioinformatics; Biomarkers; Databases; Selected reaction monitoring; Targeted proteomics.

MeSH terms

  • Biomarkers
  • Computational Biology / methods*
  • Databases, Protein
  • Peptides*
  • Proteome
  • Proteomics / methods*
  • Software
  • Web Browser


  • Biomarkers
  • Peptides
  • Proteome