Hydrodynamic HBV Transfection Mouse Model

Li-Ling Wu; Hurng-Yi Wang; Pei-Jer Chen

doi:10.1007/978-1-4939-6700-1_19

Hydrodynamic HBV Transfection Mouse Model

Methods Mol Biol. 2017:1540:227-235. doi: 10.1007/978-1-4939-6700-1_19.

Authors

Li-Ling Wu¹, Hurng-Yi Wang¹, Pei-Jer Chen²

Affiliations

¹ Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei, 100, Taiwan.
² Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei, 100, Taiwan. peijerchen@ntu.edu.tw.

PMID: 27975321
DOI: 10.1007/978-1-4939-6700-1_19

Abstract

Hydrodynamic HBV transfection mouse model is an established method of the last decade where macromolecules, non-normally permeable to cell membrane, are delivered intracellular. The basic principle is that a large volume of solution, containing HBV plasmid construct, is infused rapidly in circulation to permit the preferential entrance of these macromolecules to liver parenchymal cells. The aim of this chapter is to describe the basic principles of the hydrodynamic HBV transfection in mouse models.

Keywords: Gene transfer; HBV persistence; Hydrodynamic injection; In vivo transfection; Liver tolerance.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animal Experimentation
Animals
DNA, Viral*
Dependovirus / genetics
Genetic Vectors / administration & dosage
Genetic Vectors / genetics*
Hepatitis B virus / genetics*
Mice
Plasmids / genetics
Transfection / methods*

Substances

DNA, Viral