Engineering Fungal Nonribosomal Peptide Synthetase-like Enzymes by Heterologous Expression and Domain Swapping

Org Lett. 2016 Dec 16;18(24):6236-6239. doi: 10.1021/acs.orglett.6b02821. Epub 2016 Dec 7.

Abstract

A facile genetic methodology in the filamentous fungus Aspergillus nidulans allowed the exchange of various domains in nonribosomal peptide synthase (NRPS)-like enzymes from Aspergillus terreus. The newly generated engineered enzymes are capable of producing compounds with different chemical structures than its parent enzyme in vivo. This work provides insight in the programing of nonribosomal peptide biosynthesis in filamentous fungi.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aspergillus nidulans / enzymology*
  • Molecular Structure
  • Peptide Biosynthesis, Nucleic Acid-Independent
  • Peptide Synthases / metabolism*
  • Peptides / chemistry
  • Peptides / metabolism*
  • Protein Engineering*

Substances

  • Peptides
  • Peptide Synthases
  • non-ribosomal peptide synthase