Laser Induced breakdown spectroscopy: A rapid tool for the identification and quantification of minerals in cucurbit seeds

Jyotsana Singh; Rohit Kumar; Shikha Awasthi; Vinti Singh; A K Rai

doi:10.1016/j.foodchem.2016.10.104

Laser Induced breakdown spectroscopy: A rapid tool for the identification and quantification of minerals in cucurbit seeds

Food Chem. 2017 Apr 15:221:1778-1783. doi: 10.1016/j.foodchem.2016.10.104. Epub 2016 Oct 24.

Authors

Jyotsana Singh¹, Rohit Kumar², Shikha Awasthi³, Vinti Singh⁴, A K Rai⁵

Affiliations

¹ Centre of Food Technology, University of Allahabad, Allahabad 211002, India. Electronic address: jyotsana30@gmail.com.
² Laser Spectroscopy Research Laboratory, Department of Physics, University of Allahabad, Allahabad 211002, India. Electronic address: rohit.02dec@gmail.com.
³ Laser Spectroscopy Research Laboratory, Department of Physics, University of Allahabad, Allahabad 211002, India. Electronic address: awasthi.shi@gmail.com.
⁴ Centre of Food Technology, University of Allahabad, Allahabad 211002, India. Electronic address: vintis18@gmail.com.
⁵ Laser Spectroscopy Research Laboratory, Department of Physics, University of Allahabad, Allahabad 211002, India. Electronic address: awadheshkrai@gmail.com.

PMID: 27979160
DOI: 10.1016/j.foodchem.2016.10.104

Abstract

Laser-induced breakdown spectroscopy (LIBS) was investigated to estimate the viability as a simple and rapid method for analysis of nutrient elements in seed kernels of cucurbits. LIBS spectra were recorded in the range of 200-975nm by using Q-switched Nd:YAG laser at 532nm (4ns, 10Hz) attached to echelle spectrometer with intensified charged coupled device (ICCD). The spectral analysis revealed the presence of several elements like C, O, N, Mg, Ca, Na and K in seeds. The quantification of elements (Mg, Ca, Na and K) through LIBS was done using calibration curve method in which all calibration curve shows good linearity (r>0.95). The result obtained through LIBS was in reasonable agreement with that obtained through atomic absorption spectroscopy (AAS). Principal Component Analysis (PCA) was also applied to the LIBS data for rapid categorization of seed samples belonging to same species although samples have similar nutrient elements.

Keywords: AAS; Cucurbits; ICCD; LIBS; PCA; Seeds.

MeSH terms

Calibration
Cucurbita / chemistry*
Lasers
Minerals / analysis*
Seeds / chemistry*
Spectrophotometry, Atomic / methods*

Substances

Minerals