Photo-mediated optimized synthesis of silver nanoparticles for the selective detection of Iron(III), antibacterial and antioxidant activity

Vijay Kumar; Sweta Mohan; Devendra K Singh; Devendra K Verma; Vikas Kumar Singh; Syed Hadi Hasan

doi:10.1016/j.msec.2016.11.013

Photo-mediated optimized synthesis of silver nanoparticles for the selective detection of Iron(III), antibacterial and antioxidant activity

Mater Sci Eng C Mater Biol Appl. 2017 Feb 1:71:1004-1019. doi: 10.1016/j.msec.2016.11.013. Epub 2016 Nov 8.

Authors

Vijay Kumar¹, Sweta Mohan², Devendra K Singh², Devendra K Verma³, Vikas Kumar Singh², Syed Hadi Hasan⁴

Affiliations

¹ Nano Material Research Laboratory, Department of Chemistry, Indian Institute of Technology (BHU), Varanasi 221005, U.P., India. Electronic address: vijuevs@gmail.com.
² Nano Material Research Laboratory, Department of Chemistry, Indian Institute of Technology (BHU), Varanasi 221005, U.P., India.
³ School of Biochemical Engineering, Indian Institute of Technology (BHU), Varanasi 221005, U.P., India.
⁴ Nano Material Research Laboratory, Department of Chemistry, Indian Institute of Technology (BHU), Varanasi 221005, U.P., India. Electronic address: shhasan.apc@itbhu.ac.in.

PMID: 27987654
DOI: 10.1016/j.msec.2016.11.013

Abstract

The AgNPs synthesized by green method have shown great potential in several applications such as biosensing, biomedical, catalysis, electronic etc. The present study deals with the selective colorimetric detection of Fe³⁺ using photoinduced green synthesized AgNPs. For the synthesis purpose, an aqueous extract of Croton bonplandianum (AEC) was used as a reducing and stabilizing agent. The biosynthesis was confirmed by UV-visible spectroscopy where an SPR band at λ_max 436nm after 40s and 428nm after 30min corresponded to the existence of AgNPs. The optimum conditions for biosynthesis of AgNPs were 30min sunlight exposure time, 5.0% (v/v) AEC inoculum dose and 4mM AgNO₃ concentration. The stability of synthesized AgNPs was monitored up to 9months. The size and shape of AgNPs with average size 19.4nm were determined by Field Emission Scanning Electron Microscope (FE-SEM) and High-Resolution Transmission Electron Microscope (HR-TEM). The crystallinity was determined by High-Resolution X-ray Diffractometer (HR-XRD) and Selected Area Electron Diffraction (SAED) pattern. The chemical and elemental compositions were determined by Fourier Transformed Infrared Spectroscopy (FTIR) and Energy Dispersive X-ray Spectroscopy (EDX) respectively. The Atomic Force Microscopy (AFM) images represented the lateral and 3D topological characteristics of AgNPs. The XPS analysis confirmed the presence of two individual peaks which attributed to the Ag 3d3/2 and Ag 3d5/2 binding energies corresponding to the presence of metallic silver. The biosynthesized AgNPs showed potent antibacterial activity against both gram-positive and gram-negative bacterial strains as well as antioxidant activity. On the basis of results and facts, a probable mechanism was also proposed to explore the possible route of AgNPs synthesis, colorimetric detection of Fe³⁺, antibacterial and antioxidant activity.

Keywords: AgNPs; Antimicrobial activity; Antioxidant activity; Photoinduced route; XPS.

MeSH terms

Anti-Bacterial Agents* / chemistry
Anti-Bacterial Agents* / pharmacology
Antioxidants* / chemistry
Antioxidants* / pharmacology
Croton / chemistry*
Escherichia coli / growth & development*
Iron / analysis*
Metal Nanoparticles / chemistry*
Photochemical Processes*
Silver* / chemistry
Silver* / pharmacology
Staphylococcus aureus / growth & development*

Substances

Anti-Bacterial Agents
Antioxidants
Silver
Iron