Triazole Ureas Act as Diacylglycerol Lipase Inhibitors and Prevent Fasting-Induced Refeeding

J Med Chem. 2017 Jan 12;60(1):428-440. doi: 10.1021/acs.jmedchem.6b01482. Epub 2016 Dec 19.


Triazole ureas constitute a versatile class of irreversible inhibitors that target serine hydrolases in both cells and animal models. We have previously reported that triazole ureas can act as selective and CNS-active inhibitors for diacylglycerol lipases (DAGLs), enzymes responsible for the biosynthesis of 2-arachidonoylglycerol (2-AG) that activates cannabinoid CB1 receptor. Here, we report the enantio- and diastereoselective synthesis and structure-activity relationship studies. We found that 2,4-substituted triazole ureas with a biphenylmethanol group provided the most optimal scaffold. Introduction of a chiral ether substituent on the 5-position of the piperidine ring provided ultrapotent inhibitor 38 (DH376) with picomolar activity. Compound 38 temporarily reduces fasting-induced refeeding of mice, thereby emulating the effect of cannabinoid CB1-receptor inverse agonists. This was mirrored by 39 (DO34) but also by the negative control compound 40 (DO53) (which does not inhibit DAGL), which indicates the triazole ureas may affect the energy balance in mice through multiple molecular targets.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Eating*
  • Enzyme Inhibitors / pharmacology*
  • Fasting*
  • HEK293 Cells
  • Humans
  • Lipoprotein Lipase / antagonists & inhibitors*
  • Mice
  • Structure-Activity Relationship
  • Triazoles / chemistry*
  • Urea / chemistry*


  • Enzyme Inhibitors
  • Triazoles
  • Urea
  • Lipoprotein Lipase