cGAL, a temperature-robust GAL4-UAS system for Caenorhabditis elegans

Nat Methods. 2017 Feb;14(2):145-148. doi: 10.1038/nmeth.4109. Epub 2016 Dec 19.


The GAL4-UAS system is a powerful tool for manipulating gene expression, but its application in Caenorhabditis elegans has not been described. Here we systematically optimize the system's three main components to develop a temperature-optimized GAL4-UAS system (cGAL) that robustly controls gene expression in C. elegans from 15 to 25 °C. We demonstrate this system's utility in transcriptional reporter analysis, site-of-action experiments and exogenous transgene expression; and we provide a basic driver and effector toolkit.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Caenorhabditis elegans / genetics*
  • DNA-Binding Proteins / genetics*
  • Defecation / genetics
  • Gene Expression Regulation*
  • Genetic Engineering / methods*
  • Herpesvirus 1, Human / genetics
  • Microscopy, Fluorescence
  • Optogenetics
  • Saccharomyces cerevisiae Proteins / genetics*
  • Temperature
  • Transcription Factors / genetics*


  • DNA-Binding Proteins
  • GAL4 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors