Naa50/San-dependent N-terminal acetylation of Scc1 is potentially important for sister chromatid cohesion

Sci Rep. 2016 Dec 20;6:39118. doi: 10.1038/srep39118.


The gene separation anxiety (san) encodes Naa50/San, a N-terminal acetyltransferase required for chromosome segregation during mitosis. Although highly conserved among higher eukaryotes, the mitotic function of this enzyme is still poorly understood. Naa50/San was originally proposed to be required for centromeric sister chromatid cohesion in Drosophila and human cells, yet, more recently, it was also suggested to be a negative regulator of microtubule polymerization through internal acetylation of beta Tubulin. We used genetic and biochemical approaches to clarify the function of Naa50/San during development. Our work suggests that Naa50/San is required during tissue proliferation for the correct interaction between the cohesin subunits Scc1 and Smc3. Our results also suggest a working model where Naa50/San N-terminally acetylates the nascent Scc1 polypeptide, and that this co-translational modification is subsequently required for the establishment and/or maintenance of sister chromatid cohesion.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylation
  • Acetyltransferases / metabolism*
  • Adenosine Triphosphatases / metabolism
  • Animals
  • Cell Cycle Proteins / chemistry*
  • Cell Cycle Proteins / metabolism*
  • Cell Line
  • Cell Proliferation
  • Chromatids / genetics*
  • Chromosome Segregation
  • Drosophila Proteins / chemistry*
  • Drosophila Proteins / metabolism*
  • Drosophila melanogaster / growth & development*
  • Drosophila melanogaster / metabolism
  • Gene Expression Regulation, Developmental
  • Wings, Animal / growth & development
  • Wings, Animal / metabolism


  • Cell Cycle Proteins
  • Drosophila Proteins
  • vtd protein, Drosophila
  • Acetyltransferases
  • San protein, Drosophila
  • Adenosine Triphosphatases
  • SMC3 protein, Drosophila