Transcriptional signatures of somatic neoblasts and germline cells in Macrostomum lignano

Elife. 2016 Dec 20:5:e20607. doi: 10.7554/eLife.20607.


The regeneration-capable flatworm Macrostomum lignano is a powerful model organism to study the biology of stem cells in vivo. As a flatworm amenable to transgenesis, it complements the historically used planarian flatworm models, such as Schmidtea mediterranea. However, information on the transcriptome and markers of stem cells in M. lignano is limited. We generated a de novo transcriptome assembly and performed the first comprehensive characterization of gene expression in the proliferating cells of M. lignano, represented by somatic stem cells, called neoblasts, and germline cells. Knockdown of a selected set of neoblast genes, including Mlig-ddx39, Mlig-rrm1, Mlig-rpa3, Mlig-cdk1, and Mlig-h2a, confirmed their crucial role for the functionality of somatic neoblasts during homeostasis and regeneration. The generated M. lignano transcriptome assembly and gene expression signatures of somatic neoblasts and germline cells will be a valuable resource for future molecular studies in M. lignano.

Keywords: Macrostomum; developmental biology; flatworms; germline; regeneration; stem cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Gene Expression Profiling
  • Germ Cells / physiology*
  • Platyhelminths / cytology*
  • Platyhelminths / genetics*
  • Stem Cells / physiology*
  • Transcriptome*

Grants and funding

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.