miR-24-3p regulates bladder cancer cell proliferation, migration, invasion and autophagy by targeting DEDD

Oncol Rep. 2017 Feb;37(2):1123-1131. doi: 10.3892/or.2016.5326. Epub 2016 Dec 16.


microRNAs (miRNAs), a class of small non-coding RNA molecules, can regulate gene expression by interacting with the 3'-untranslated regions (3'UTR) of target genes and influence various biological processes. We investigated the potential role of miR-24-3p in the development of bladder cancer by regulating DEDD, a member of the death effector domain-containing protein family. First, we found that miR-24-3p was highly expressed and that DEDD was expressed at a low level in bladder cancer tissues compared with that in adjacent bladder tissues by qRT-PCR (P<0.0001). Second, we found that miR-24-3p promoted the proliferation ability of bladder cancer cells using the MTT assay and colony forming assay; and showed that miR-24-3p accelerated the migration and invasion of bladder cancer cells using migration and invasion assays (P<0.05). Moreover, miR-24-3p inhibited apoptosis of bladder cancer cells, as shown by flow cytometry (P<0.05). Western blot results demonstrated that miR-24-3p participated in autophagy of bladder cancer cells by DEDD. In addition, the tumor formation assay showed that miR-24-3p promoted the growth of bladder tumor in vivo. Furthermore, the luciferase reporter gene assay indicated that miR-24-3p suppressed DEDD gene transcription. Therefore, our study indicated that miR-24-3p promoted bladder cancer progression by inhibiting DEDD.

MeSH terms

  • 3' Untranslated Regions
  • Animals
  • Apoptosis / genetics
  • Autophagy / genetics*
  • Cell Line, Tumor
  • Cell Movement / genetics
  • Cell Proliferation / genetics
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Death Domain Receptor Signaling Adaptor Proteins / genetics*
  • Death Domain Receptor Signaling Adaptor Proteins / metabolism
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Mice, Inbred BALB C
  • MicroRNAs / genetics*
  • Microtubule-Associated Proteins / genetics
  • Microtubule-Associated Proteins / metabolism
  • Urinary Bladder Neoplasms / genetics
  • Urinary Bladder Neoplasms / pathology*
  • Xenograft Model Antitumor Assays


  • 3' Untranslated Regions
  • DEDD protein, human
  • DNA-Binding Proteins
  • Death Domain Receptor Signaling Adaptor Proteins
  • MAP1LC3A protein, human
  • MIRN24 microRNA, human
  • MicroRNAs
  • Microtubule-Associated Proteins