Effect of freeze-thaw process on physical properties, microbial activities and population structures of anaerobic sludge

Chantaraporn Phalakornkule; Sasikarn Nuchdang; Maneerat Khemkhao; Wuttichai Mhuantong; Sarunyou Wongwilaiwalin; Sithichoke Tangphatsornruang; Verawat Champreda; Jiraprat Kitsuwan; Savitri Vatanyoopaisarn

doi:10.1016/j.jbiosc.2016.11.005

Effect of freeze-thaw process on physical properties, microbial activities and population structures of anaerobic sludge

J Biosci Bioeng. 2017 Apr;123(4):474-481. doi: 10.1016/j.jbiosc.2016.11.005. Epub 2016 Dec 19.

Authors

Chantaraporn Phalakornkule¹, Sasikarn Nuchdang², Maneerat Khemkhao³, Wuttichai Mhuantong⁴, Sarunyou Wongwilaiwalin⁴, Sithichoke Tangphatsornruang⁵, Verawat Champreda⁴, Jiraprat Kitsuwan⁶, Savitri Vatanyoopaisarn⁷

Affiliations

¹ The Research and Technology Center for Renewable Products and Energy, King Mongkut's University of Technology North Bangkok, Bangsue, Bangkok 10800, Thailand; Department of Chemical Engineering, Faculty of Engineering, King Mongkut's University of Technology North Bangkok, Bangsue, Bangkok 10800, Thailand. Electronic address: cpk@kmutnb.ac.th.
² Thailand Institute of Nuclear Technology, Nakhon Nayok 26120, Thailand.
³ The Research and Technology Center for Renewable Products and Energy, King Mongkut's University of Technology North Bangkok, Bangsue, Bangkok 10800, Thailand.
⁴ Enzyme Technology Laboratory, Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology, Thailand Science Park, Khlong Luang, Pathum Thani 12120, Thailand.
⁵ Genome Institute, National Center for Genetic Engineering and Biotechnology, Thailand Science Park, Khlong Luang, Pathum Thani 12120, Thailand.
⁶ The Research and Technology Center for Renewable Products and Energy, King Mongkut's University of Technology North Bangkok, Bangsue, Bangkok 10800, Thailand; Department of Chemical Engineering, Faculty of Engineering, King Mongkut's University of Technology North Bangkok, Bangsue, Bangkok 10800, Thailand.
⁷ Department of Agro-Industrial Technology, Faculty of Applied Science, King Mongkut's University of Technology North Bangkok, Bangsue, Bangkok 10800, Thailand.

PMID: 28007421
DOI: 10.1016/j.jbiosc.2016.11.005

Abstract

Effects of the freeze-thaw process on physical properties, cell viability, microbial activities and population structures of anaerobic sludge were investigated. It was found that the sludge volume index was greatly reduced from 16.4 mL/g in the original sludge to 4.0 mL/g in the solid fraction of the frozen-thawed sludge. Even though the freeze-thaw process decreased cell viability in the solid fraction of the frozen-thawed sludge, microbial activity tests showed that the freeze-thaw process enhanced acidogenic activity approximately 20%. The enhanced acidogenic activity of the solid fraction was in good agreement with the enrichment of Clostridiaceae, Porphyromonadaceae and Propionibacteriaceae found in the solid fraction. The relative abundances of Proteobacteria families Oxalobacteraceae, Moraxellaceae, and Pseudomonadaceae were found to be highest in the liquid fraction where they form a substantial proportion of the bacterial community (a total of 59%).

Keywords: Anaerobic sludge; Biohydrogen; Freeze–thaw; Microbial activity; Tagged 16S rRNA gene pyrosequencing.

MeSH terms

Acids / metabolism
Anaerobiosis
Freezing*
Hydrogen-Ion Concentration
Microbial Viability
Proteobacteria / genetics
Proteobacteria / isolation & purification
Proteobacteria / metabolism
RNA, Ribosomal, 16S / genetics
Sewage / microbiology*

Substances

Acids
RNA, Ribosomal, 16S
Sewage