Response to Comment on "Structural basis of histone H3K27 trimethylation by an active polycomb repressive complex 2"

Science. 2016 Dec 23;354(6319):1543. doi: 10.1126/science.aaj2335.

Abstract

Zhang et al suggested that in the crystal structure of a polycomb repressive complex 2 from Chaetomium thermophilum (ctPRC2), a flexible linker region, but not the H3K27M cancer mutant peptide, better fits the electron density. Based on our new data, we agree with this alternative interpretation and provide the crystal structure of ctPRC2 bound to a bona fide H3K27M sequence.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Comment

MeSH terms

  • Chaetomium / metabolism
  • Histones / chemistry*
  • Humans
  • Lysine / metabolism
  • Polycomb Repressive Complex 2 / chemistry*

Substances

  • Histones
  • Polycomb Repressive Complex 2
  • Lysine