Chemical Fingerprint Analysis and Quantitative Analysis of Rosa rugosa by UPLC-DAD

Molecules. 2016 Dec 21;21(12):1754. doi: 10.3390/molecules21121754.


A method based on ultra performance liquid chromatography with a diode array detector (UPLC-DAD) was developed for quantitative analysis of five active compounds and chemical fingerprint analysis of Rosa rugosa. Ten batches of R. rugosa collected from different plantations in the Xinjiang region of China were used to establish the fingerprint. The feasibility and advantages of the used UPLC fingerprint were verified for its similarity evaluation by systematically comparing chromatograms with professional analytical software recommended by State Food and Drug Administration (SFDA) of China. In quantitative analysis, the five compounds showed good regression (R² = 0.9995) within the test ranges, and the recovery of the method was in the range of 94.2%-103.8%. The similarities of liquid chromatography fingerprints of 10 batches of R. rugosa were more than 0.981. The developed UPLC fingerprint method is simple, reliable, and validated for the quality control and identification of R. rugosa. Additionally, simultaneous quantification of five major bioactive ingredients in the R. rugosa samples was conducted to interpret the consistency of the quality test. The results indicated that the UPLC fingerprint, as a characteristic distinguishing method combining similarity evaluation and quantification analysis, can be successfully used to assess the quality and to identify the authenticity of R. rugosa.

Keywords: Rosa rugosa; UPLC-DAD; fingerprint; quality control.

Publication types

  • Validation Study

MeSH terms

  • China
  • Chromatography, High Pressure Liquid / instrumentation
  • Chromatography, High Pressure Liquid / methods
  • Chromatography, High Pressure Liquid / standards*
  • Drugs, Chinese Herbal / chemistry
  • Drugs, Chinese Herbal / standards*
  • Ellagic Acid / isolation & purification
  • Ellagic Acid / standards
  • Gallic Acid / isolation & purification
  • Gallic Acid / standards
  • Humans
  • Kaempferols / isolation & purification
  • Kaempferols / standards
  • Quality Control
  • Quercetin / analogs & derivatives
  • Quercetin / isolation & purification
  • Quercetin / standards
  • Reproducibility of Results
  • Rosa / chemistry*
  • Sensitivity and Specificity


  • Drugs, Chinese Herbal
  • Kaempferols
  • Ellagic Acid
  • kaempferol 3-O-sophoroside
  • Gallic Acid
  • hyperoside
  • Quercetin
  • astragalin